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However, TMAO insult alone was not sufficient to convert renal fibroblasts to myofibroblasts requiring synergy with a preexisting pathological state, i.e., pro-fibrotic signaling (second-hit) in the form of TGF-1 stimulation, the most prominent pro-fibrotic cytokine 46

Involvement of ROS is implicated in neurodegenerative and other disorders such as Alzheimers disease, Parkinsons disease, multiple sclerosis, Down syndrome, inflammation, viral infection, autoimmune pathology, and digestive ulcers

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This discrepancy might be attributable to methodological factors, such as the testing duration