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Heme-Rbt and heme-HuHPX Affi-Gel 15 affinity-isolated proteins were fractionated using SDS-PAGE electrophoresis, transferred to PVDF membranes, and biotinylated proteins detected using streptavidinHRP

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The phenolic ratios in the composition may differ based on the age of the plant, growing conditions, quantification method, and extraction technique and solvent, even though there are numerous findings on the phenolic composition of AVE in the literature