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High-fat diet enhances stemness and tumorigenicity of intestinal progenitors

Many of these products are manufactured without strict quality controls, leading to issues with purity issues, heavy metal contamination, or the presence of residual TFA (trifluoroacetic acid) from the synthesis process

A volume of 5 l of the protein-dye mixture was dispensed into a 384-well microplate (Thermo Scientific, AB1384K), followed by addition of 5 l of C1-27 and 3 l of silicone oil (to avoid evaporation)