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Cell growth assay Cells were plated at 2,500 cells per well in 96-well plates for calcein-AM staining or 7,500 cells per well in 48-well plates for cell counting

It further exerts immune-modulatory effects by reducing TNF-, IL-6, and NF-B activity, thus favoring an anti-tumor immune milieu, which further increases the responsiveness of chemotherapy and immunotherapy (Mohan et al., 2004)

The conclusion described directions for future rooibos research to establish its activity and utility as a human chemopreventive and therapeutic agent

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