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Together these results demonstrated that fumarate, when accumulated, leads to GSH succination

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Secondary antibodies used were horseradish peroxidase-linked anti-rabbit (CST, ##7074, 1:5000 dilution), horseradish peroxidase-linked anti-mouse (CST, #7076, 1:5000 dilution), DyLight-800 4 PEG-linked anti-rabbit (CST, #5151, 1:5000 dilution), and DyLight 680-linked anti-mouse (CST, #5470, 1:5000 dilution)

Further exploration revealed that this was driven mainly by the difference between patients with ME/CFS and HCs (F = 9.589, p = 0.004), with ME/CFS patients showing higher lactate levels than controls, although a trend towards significant difference between patients with ME/CFS and long COVID was also noted (F = 4.031, p = 0.052)