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We therefore used EMS mutagenesis to isolate 14 independent strains that were resistant to 5 mM DTT and analysed nine of these using a combined SNP-based mapping and whole-genome re-sequencing protocol [13]

Common techniques include click chemistry (e.g., azide-alkyne reactions), thiol-maleimide coupling, and EDC/NHS-mediated amide bond formation, all of which exploit naturally occurring or introduced reactive groups like amines or thiols on EV surfaces (Fig

Cloudiness or precipitate indicates incomplete dissolution or contamination

Our results demonstrate that GLUT3 plays a critical role in promoting both allergic inflammation and wound healing functions of macrophages