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In preclinical research models, exposure to GHK-Cu has been associated with altered gene expression patterns and modulation of signalling pathways related to extracellular matrix dynamics, oxidative stress response, and cellular turnover

For parthanatos, DNA damage caused by mitochondrial impairment activates poly(ADP-ribose) polymerase 1 (PARP1), and excessive production of poly(ADP-ribose) (PAR) leads to NAD+ depletion and mitochondrial function collapse, ultimately inducing parthanatos [42]

( d-e ) Western blotting for total and phospho (p-)ERK1/2 levels in ( d ) SLC31A1-KO or ( e ) BCS treated SEM, NALM6, REH or MOLT16 cells

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