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control solution and sample solution was pipetted precisely respectively in 5 ml volumetric flask, for each one, 100 l of 0.05 mol/L carbonate buffer solution (4.2 g sodium bicarbonate was dissolved in 900 ml water, pH was adjusted to 8.4 with 5 mol/L NaOH), 100 l of pyridine acetonitrile solution (per 1 ml acetonitrile contains 25 l of pyridine) and 200 l of derivatization reagent solution (0.5% (+) -methyl-6-methoxy-2-naphthyl acetyl chloride) was mixed, sealed and reacted at 40 C

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