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For in vitro binding experiments of mSIN with AKT, equal amounts of the purified MBP or MBP-mSIN proteins immobilized to dextran sepharose TM 4B beads were incubated with bacterially purified GST-AKT (0.5 g) either in the presence of recombinant His-PTPN22 or equal volume of corresponding buffer for 1 h at 4 C

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Observational | Animal | In vitro Stable gastric pentadecapeptide BPC 157: protective effects and healing mechanisms Animal | In vitro What is BPC-157