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After treatment with HRP blocker for 15 min and washing with wash buffer for 2 min, nuclear staining was performed with DAPI, and the slides were mounted with fluorescence mounting medium

That enzyme is NNMT, nicotinamide N-methyltransferase

This sensitivity is essential for the bodys ability to respond appropriately to the presence of stool in the rectum, thus preventing involuntary leakage and maintaining continence

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