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Generation of mutant mice by CRISPR-mediated genome editing To generate a null mutant of Vmn2r88 , we designed two guide RNAs (gRNAs) for each gene that were able to introduce double-strand DNA breaks flanking exon 3-6 of the V2R gene in which the essential transmembrane domain is encoded

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Our analyses revealed that the shared methylated genes were involved in multiple BPs and MFs (Supplementary File S6)