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For liquid media, we used Brucella Broth (BBL) media supplemented with 10% heat-inactivated fetal bovine serum (FBS, Life Technologies) (BB10)
Trends Plant Sci 19:623630 Guo P, Baum M, Grando S, Ceccarelli S, Bai G, Li R, von Korff M, Varshney RK, Graner A, Valkoun J (2009) Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage
However, this method is more invasive and carries a higher risk of infection
