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For the other immune molecules, the expression level of those genes reached the highest level in stage C or stage D and the lowest level in E stage or AB stage, such as metallothionein , metallothionein 2 , macrophage migration inhibitory factor , macrophage migration inhibitory factor MIF1 , hemocyanin subunit 1 , hemocyanin subunit 2 , and hemocyanin subunit 5

In Acute Diseases: Horses and Cattle : 10.0 25.0 ml / Animal / day Foals and Calves : 5.0 12.0 ml / Animal / day Sheep and Goats : 2.5 5 ml / Animal / day Lambs and kids : 1.5 2.5 ml / Animal / day Dogs : 0.5 5.0 ml / Animal / day Cats : 0.5 2.5 ml / Animal / day Poultry : 1-3 ml / liter of drinking water To be repeated if required

2 h post-infection, cells were washed with cold PBS and lysed with TBS buffer containing 150 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 1% Triton X-100, following with 10% sonication for 10 s